WebAug 18, 2024 · Co-targeting of O-GlcNAc transferase (OGT) and the transcriptional kinase cyclin-dependent kinase 9 (CDK9) is toxic to prostate cancer cells. As OGT is an essential glycosyltransferase, identifying an alternative target showing similar effects is of great interest. Here, we used a multiomics approach (transcriptomics, metabolomics, and ... WebNov 18, 2024 · Accordingly, co-targeting of CDK7 and CDK9 synergistically blocks the proliferation of the CRPC cells but does not have anti-proliferative effects in the normal prostate cells. Conclusion. We show that CRPC cells, but not normal prostate cells, are addicted on the high activity of the key transcriptional kinases, CDK7 and CDK9.
Transcriptional programming drives Ibrutinib …
WebJun 19, 2024 · CDK9 activity is required to maintain the phosphorylation status of Spt5 upstream of the poly(A) site, most likely by preventing Spt5 dephosphorylation by PP1. Indeed, PP1 is a target of CDK9 in both yeast and human [30, 31] and this phosphorylation is inhibitory [30, 64]. Thus, CDK9 neutralizes PP1 activity during elongation, thereby ... WebNov 15, 2024 · Immunoprecipitation was performed using an antibody against CDK9, and the respective co-precipitation Cyclin T1 was assessed using Western Blot. (F) … self neglect and the care act
MYC protein interactors in gene transcription and cancer
WebFeb 2, 2024 · Cyclin-dependent kinase 9 (CDK9) is a key transcriptional regulator and promising prognostic marker and therapeutic target in cancers. 16,17 Unlike other CDKs, CDK9 does not regulate the cell cycle but promotes RNA synthesis in the genetic processes of cell growth, differentiation and viral pathogenesis. 18–20 Previous data indicated that … WebSep 15, 2024 · Small-molecule drugs targeting the CDK9 kinase, dinaciclib, flavopiridol, roscovitine, AT-7519, SNS-032, and DRB, diminished MDM4 levels and activated p53 in A375 melanoma and MCF7 breast ... WebMar 14, 2024 · We co-overexpressed FLAG-CDK9 with HA-mLST8, HA-RAPTOR, MYC-RICTOR, MYC-mSIN1.1, or YFP-mTOR in 293T cells and assessed the ability of CDK9 to bind different mTOR complex components by co-IP. CDK9 interacted with all the main mTORC1 and mTORC2 pathway components (Figure 2C-E), suggesting an involvement … self neglect cqc