Gapdh antibody dilution
WebSee all GAPDH antibodies. Applications Immunofluorescence analysis of GAPDH (green) showing staining in the cytoplasm of HeLa cells (right), compared with a negative control … WebMar 31, 2024 · The antibody dilutions were 800-fold in most cases except for GAPDH (5000-fold dilution), B-ACT and ALB (2000-fold dilution). Washing 5 times with T-TBS (200 µL/well). Incubation with secondary antibody 4000-fold diluted with blocker for 1 h at 37 °C with 400 rpm in the orbital shaker (100 µL/well).
Gapdh antibody dilution
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WebMy GAPDH is a rabbit polyclonal antibody, using dilution of 1:20000. But for secondary antibody, I used 1:5000. Sometimes I wonder would it be the cross-activity across the species. WebGAPDH作为管家基因在同种细胞或者组织中的蛋白质表达量一般是恒定的，且几乎在所有组织中都高水平表达，因此被广泛用作Western blot蛋白质标准化的内参。. 产品编号. 产品名称. 包装. AG0122. GAPDH Rabbit Monoclonal Antibody. 50µl. AZ050. Western一抗稀释液.
WebThe cells were labeled with GAPDH Mouse Monoclonal Antibody (Product # AM4300) at 1 µg/mL in 1% BSA and incubated for 3 hours at room temperature and then labeled with … WebGAPDH作为管家基因在同种细胞或者组织中的蛋白质表达量一般是恒定的，且几乎在所有组织中都高水平表达，因此被广泛用作Western blot蛋白质标准化的内参。. 产品编号. 产品 …
WebApr 8, 2024 · The end-point dilution, where the OD450 score was more than 2-fold of the OD450 of the background control wells, was determined as the titer of anti-CVA10 IgG. 2.9. Virus Neutralization Assay. ... 48, and 72 h.p.i. by anti-CVA6/CVA10 and GAPDH antibodies, respectively. The symbol **** was used to indicate p < 0.0001, respectively. … WebGet better batch-to-batch reproducibility with a recombinant antibody Anti-GAPDH antibody [EPR16891] - Loading Control (ab181602) Research with confidence – …
WebA specific band was detected for GAPDH at approximately 43 kDa (as indicated) using 10 µg/mL of Goat Anti-Human/Mouse/Rat GAPDH Antigen Affinity-purified Polyclonal Antibody (Catalog # AF5718) followed by 1:50 dilution of HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF109). This experiment was conducted under reducing …
WebThe GAPDH (glyceraldehyde-3-phosphate dehydrogenase) gene is mapped to human chromosome 12p13.3 and encodes a tetramer containing identical chains. 1] The enzyme is commonly known as glycolytic enzyme and contains a binding site for NAD + (nicotinamide adenine diphosphate) and glyceraldehyde-3-phosphate. GAPDH contributes to 10-20% … one chance yeah hoiWebThe membrane was probed with a GAPDH polyclonal antibody (Product # PA1-987) at a dilution of 1:3000 overnight at 4°C, washed in TBST, and probed with an HRP-conjugated secondary antibody for 1 hr at room … is baclofen or methocarbamol strongerone change mangoWebRecommended dilution: WB 1:10000 - 1:760000; IHC 1:100 - 1:500; IF/ICC 1:50 - 1:200; Antibody Dilute Procedure: Put 5ul AC033 into 45 ul buffer(with 50% glycerol), diluted … one change in people\u0027s personal relationshipWebGAPDH antibody used for WB at 1:1000 dilution on lysates of mouse embryonal carcinoma P19 cell line which was induced to differentiate for 5 days while being … one chance to save the world gameWebAnti-GAPDH Antibody. Anti-Glyceraldehyde-3-Phosphate Dehydrogenase Antibody is popular affinity purified polyclonal antibody. This antibody is supported by peer reviewed publications and reliably detects Glyceraldehyde-3. Anti-GAPDH Antibody MSDS (material safety data sheet) or SDS, CoA and CoQ, dossiers, brochures and other available … is baclofen ototoxicWebImmunocytochemistry analysis of GAPDH in HeLa cells. Samples were incubated in GAPDH monoclonal antibody ( Product # MA1-16757) using a dilution of 1:100. … one change place