WebBCA™ Reagent A, 250 ml, contains sodium carbonate, sodium bicarbonate, bicinchoninic acid and sodium tartarate in 0.1 M sodium hydroxide BCA™ Reagent B, 25 ml, contains 4% cupric sulfate Compatibility Reagent, 10 × 20 mg Reconstitution Buffer, 15 ml Albumin Standard, 2 mg/ml, 10 × 1 ml ampules, contains bovine serum albumin at 2.0 mg/ml in WebUrinalysis using a dipstick reagent strip is an effective screening tool to assess the health status of an individual and detect some diseases and infections. It is impor-tant that …
BCA Protein Assay Kit - Cell Signaling Technology
WebOct 26, 2016 · Keller and Nellville[23] have examined the BCA, Biuret, Bio-Rad Coomassie Blue and Pierce BCA methods for the assay of total protein in human milk. Though there was a good correlation between the values obtained from the colorimetric assays with the standard micro-Kjehdahl method, only BCA assay displayed the greatest accuracy and … Web23231 Micro BCA™ Reagent A (MA), 240 ml, containing sodium carbonate, sodium bicarbonate and sodium tartrate in 0.2 N NaOH 23232 Micro BCA™ Reagent B (MB), 240 ml, containing bicinchoninic acid (4.0%) in water 23234 Micro BCA™ Reagent C (MC), 12 ml, containing 4.0% cupric sulfate, pentahydrate in water birnbaum\\u0027s walt disney world
Protein quantitation assay compatibility table - Thermo Fisher …
WebB-PER reagent Undiluted Undiluted 1:3 NA NA Undiluted 1:2 1:2 1:2 Undiluted B-PER reagent II NA NA 1:4 NA NA NA 1:4 NA 1:2 Undiluted ... Micro BCA Pierce Modified Lowry Pierce Detergent Compatible Bradford Pierce Coomassie Plus Pierce Coomassie Pierce 660 nm Qubit Protein BR Assay Web2. Prepare WR by mixing 50 parts Rapid Gold BCA Reagent A with 1 part Rapid Gold BCA Reagent B (50:1, Reagent A:B). For the above example, combine 10 mL of Reagent A with 0.2 mL of Reagent B. Note: When Rapid Gold BCA Reagent B is first added to Rapid Gold BCA Reagent A, a pale blue precipitate may be observed, but, upon WebMay 26, 2024 · Calculate the amount of Micro BCA reagents required (provided in the kit) based on number of wells that require reagents. For example, for 60 wells (including 16 wells for standards) use as follows: Micro BCA™ Reagent A: 50 μL × 60 = 3000 μL, Micro BCA™ Reagent B: 48 μL × 60 = 2880 μL, Micro BCA™ Reagent C: 2 μL × 60 = 120 μL. birth by sleep tropes