WebAnti-Podocin antibody produced in rabbit (affinity isolated antibody, buffered aqueous solution); Suitable for western blot (chemiluminescent); Anti-Podocin antibody produced in rabbit has been used for Western Blotting and immunohistochemistry; Podocin is a podocyte structural protein, that belo
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WebRabbit monoclonal (Y17-R) antibody to Nephrin. This antibody recognizes the epitope located between Cys465 - Cys528. WB: 1:5,000, ELISA: 1:50,000-1:100,000. Peptide derived from the centre of the fifth Ig-like domain of human nephrin. Supplied in Tris Buffered Saline, pH 8.0, with 10 mg/ml BSA and 0.05% Sodium Azide. WebThus, Nephrin may constitute the entire extracellular structure of the slit-diaphragm (2,3). Application Note. Anti-Nephrin Antibody has been tested for use in ELISA, Western Blotting, immunofluorescence, and immunohistochemistry. Expect a band at approximately 135 kDa in Western Blots of specific cell lysates and tissues.
WebNephrin Antibodies. (Nephrosis 1, Congenital, Finnish Type (Nephrin) (NPHS1)) This gene encodes a member of the immunoglobulin family of cell adhesion molecules that functions in the glomerular filtration barrier in the kidney. The gene is primarily expressed in renal tissues, and the protein is a type-1 transmembrane protein found at the slit ... WebValidations: None Available. Applications: WB, IHC and 2 more. Reactivity: Homo sapiens (Human), Mus musculus (House mouse), and Rattus norvegicus (Rat) Nephrin Antibody - BSA Free. NBP1-77303 is a primary antibody from Novus Biologicals.
WebJan 23, 2006 · Time courses of serum anti-nephrin antibody titers of rats immunized with different fragments of nephrin cDNA. GST fusion proteins produced by E. coli BL21 were used as antigens for ELISA. IgG anti-nephrin antibody titers against Ig-like motifs 1–2, 3–4, 5–6 and 7–8 of nephrin protein are shown in panels A, B, C and D, respectively. WebSep 1, 2024 · The effect of topiroxostat in anti-nephrin antibody (ANA)-induced podocyte injury. (a) Representative IF findings (upper panel), and the semi-quantitative data of immunostaining (lower panel) of podocyte functional molecules. Nephrin, podocin and podoplanin were observed as a continuous linear pattern along capillary loop in normal …
WebMar 21, 2024 · KIRREL1 (Kirre Like Nephrin Family Adhesion Molecule 1) is a Protein Coding gene. Diseases associated with KIRREL1 include Nephrotic Syndrome, Type 23 and Nephrotic Syndrome . Among its related pathways are Cell junction organization and Nephrin family interactions . An important paralog of this gene is KIRREL3.
WebAlexafluor phalloidin (peripheral and stress fibre patterning) was used to stain the actin cytoskeleton and an anti-nephrin antibody (cytoplasmic localisation) was used to detect the slit-diaphragm protein nephrin. Nephrin had a predominant cytoplasmic distribution in all conditions. The cells had a variable appearance from rounded to spread. closing out a joint checking accountWebNephrin Antibodies. Antibodies that detect Nephrin can be used in several scientific applications, including Western Blot, Immunohistochemistry, Immunocytochemistry, ELISA and Dot blot. These antibodies target Nephrin in Human, Mouse and Rat samples. Our Nephrin polyclonal and monoclonal antibodies are developed in Rabbit and Mouse. closing out an emailWebNephrin not only functions as the core component of the extracellular SD filtration network, ... 1193, and 1217 is the most widely characterized, potentially due to the commercial availability of phospho-specific antibodies for these sites . Phosphorylation at these sites results in the recruitment of Nck, PLC-γ1, ... closing out an essayWebJun 20, 2024 · Antibodies and immunoblotting. Purified rabbit polyclonal antibodies against nephrin, p-nephrin antibodies have been described previously [14–17].β-actin and synaptopodin antibodies were obtained from Sigma-Aldrich.Proteins were extracted from plasma membranes in RIPA buffer (PBS containing 0.1% SDS, 1% Nonidet P-40, 0.5% … closing out an agile projectWebAug 3, 2024 · nephrin Antibody (G-8) is a mouse monoclonal IgG 2a κ nephrin antibody, cited in 24 publications, provided at 200 µg/ml. raised against amino acids 23-322 mapping within an extracellular domain of nephrin of rat and human origin. nephrin Antibody (G-8) is recommended for detection of nephrin of mouse, rat and human origin by WB, IP, IF … closing out an estate accountWebSep 30, 2024 · We report our experience with a C57BL/6 FSGS model induced by polyclonal rabbit anti-mouse nephrin antibody (α-mNep Ab). Methods: α-mNep Ab, which was generated by genetic immunization, was ... closing out an estate bank account in paWebAll lanes : Anti-Nephrin antibody [EPR20993] (ab216341) at 1/1000 dilution Lane 1 : Mouse kidney lysate Lane 2 : Mouse spleen lysate Lysates/proteins at 10 µg per lane. … closing out an estate in nc