WebJan 3, 2016 · Analysis by the PCR/ESI- MS method using nine primer pairs targeting the most information-rich regions of the trpE, adk, mutY, fumC, and ppa genes distinguished 47 of the 48 A. baumannii genotypes ... WebOct 17, 2012 · My interest relies on the study of microbial ecology in human related environment such as gut microbiota. Microbial genomics, metagenomics and cytometry-based single cell genomics are my current working field. I have expertise in de-novo genome sequencing, assembly and finishing. Trying to put order in the genomic …
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The overlap extension polymerase chain reaction (or OE-PCR) is a variant of PCR. It is also referred to as Splicing by overlap extension / Splicing by overhang extension (SOE) PCR. It is used assemble multiple smaller double stranded DNA fragments into a larger DNA sequence. OE-PCR is widely used to insert … See more As in most PCR reactions, two primers—one for each end—are used per sequence. To splice two DNA molecules, special primers are used at the ends that are to be joined. For each molecule, the primer at the end to … See more To insert a mutation into a DNA sequence, a specific primer is designed. The primer may contain a single substitution or contain a new sequence at its 5' end. If a deletion is … See more Besides the introduction of mutations, Overlap Extension PCR is widely used to assemble complex DNA sequences without the … See more WebApr 10, 2024 · Schematic representation of overlap extension PCR to create specific nucleotide at the target site of pvmdr1 gene. To construct a mutant control allele for … thunder sakura locations genshin impact