WebbOur study aimed at investigating the turnover of these mucins in the small intestine by using in vivo labeling of O-glycans with N-azidoacetylgalactosamine. Mice were injected intraperitoneally and sacrificed every hour up to 12 hours and at 24 hours. Samples were fixed with preservation of the mucus layer and stained for Muc2 and Muc17. Webb1 maj 2024 · SARS-CoV-2 infects airway and gut organoids. Organoids are three-dimensional (3D) structures that can be grown from adult stem cells and recapitulate key aspects of the organ from which those cells derive. Because SARS-CoV and SARS-CoV-2 target the lung, we added virus to organoid-derived human airway epithelium cultured in …
A small intestinal organoid model of non-invasive enteric …
WebbUses mouse organoids, we showed that IL-10 modulates chalice cell frequencies in mammals, suggesting conservation across species. Dieser study demonstrates a previously unappreciated IL-10-Notch axis regulating goblet cell homeostasis in aforementioned developing zebrafish intestine and may aid explain the disease grade … WebbSmall intestinal organoids were the first established organoids with proliferative ... In addition to cost reduction, type I collagen is physiologically relevant as a major ECM in the small intestine 44, ... (1:1000) for 10 min at RT. Fluorescence staining was visualized with fully focused z-stack images using an all-in-one fluorescence ... blackmagic hdmi converter
Protocol Guide: Immunofluorescent Staining of Whole …
WebbImmunofluorescence staining of organoids. Immunofluorescence staining of organoids was performed as previously described (Serra et al. 2024). Briefly, the contents of each well plate were centrifuged at 3000 rpm for 10 min at 10 °C before fixation to allow imaging of organoids within a similar z-range. Webb31 juli 2024 · Radiation-induced intestinal injuries (RIII) commonly occur in patients who suffer from pelvic or abdominal cancer. Nuclear factor-erythroid 2-related factor 2 (Nrf2) is a key transcriptional regulator of antioxidant, and the radioprotective role of Nrf2 is found in bone marrow, lung, and intestine, etc. Here, we investigated the effect of Nrf2 knockout … Webb8 jan. 2024 · The derivation of organoids from intestinal tissues revealed a new way to mimic the complex organization and functionality of tissues in vivo. 1 Remarkably, … gaps phonics intervention