Thp1 il-1β
WebInvivoGen has developed a cell-based assay to monitor the activation of the NLRC4 inflammasome by intracellular flagellin. This assay relies on two cell lines: • THP1-NLRC4 … Web1.1 材料 人单核细胞(THP-1细胞)购自美国ATCC；DMSO培养基、佛波酯(PMA)购自美国 Sigma公司；FBS、EDTA购自美国Gibcog公司；吡格列酮(Piog，PPAR-γ激动剂) …
Thp1 il-1β
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WebAug 1, 2011 · Pro-IL-1β is cleaved by the pro-inflammatory protease caspase-1 [3].The activation of caspase-1 occurs via recruitment to a multi-protein complex termed the … WebIL-1β levels by Flow Cytometry When THP-1 cells were stimulated with 100 ng/mL E. coli LPS, the percentage of IL-1β was found to be 36.7%, and this value decreased to 1.7 after …
WebApr 30, 2024 · THP-1 cells (5-6 × 10 6 cells/ml) were labeled with 10 μM calcein AM (Sigma-Aldrich, USA) for 45 min at 37° C. After the stimulation, THP-1 cells were added to HAECs … Webproduction of pro-IL-1β, the immature form of IL-1β. Subsequent stimulation with inflammasome inducers, such as ATP and alum crystals, leads to caspase-1 activation …
WebEscherichia coli LPS was used as the control. In this study, P. gingivalis LPS showed the ability to induce cytokine production in THP-1 cells and its induction was significantly (P < … WebPlus particulièrement, les polymorphismes génétiques de l’IL-1β (IL-1β -31 et -511) ne semblent pas influencer l’expression de l’IL-1β de manière significative.Au regard des ...
WebRecombinant purified HP1173 (rHP1173) was found to bind to THP-1 cells that were differentiated into macrophages via phorbol-12-myristate-13-acetate (PMA) treatment. …
WebAug 19, 2016 · Caspase-1 activation and IL-1β production from THP-1 cells by Western blotting and the culture supernatant by enzyme-linked immunosorbent assay analysis … birthday gifts for my adult goddaughterWebMar 4, 2024 · THP-1-defCASP1 cells demonstrated significant reductions in IL-1β expression in comparison to wild-type THP-1 cells upon infection with both early and … danner east ridge bootsdanner east ridge boots used for hikingWebSep 27, 2024 · Optimal response to LPS occurs when the CD14 cell surface protein is also present and is characterized by the production of the pro-inflammatory interleukin-8 (IL-8) … danner factory store portlandTo further investigate whether RIG-I can affect EV-A71-induced IL-1β release in an NLRP3-independent manner, NLRP3 KD THP-1 macrophages were transfected with siRIG-I and then infected with EV-A71. The expression levels of RIG-I, NLRP3, and EV-A71 VP0 were confirmed by Western blotting (Fig. 3A). The … See more TLRs are known to be implicated in pathogen-induced IL-1β production31. Among these TLRs, evidence suggests that TLR3 and TLR8 play roles in RNA virus … See more TLR engagement has been demonstrated to be sufficient to promote inflammasome activation in human monocytes. To test the effects of TLR3 and TLR8 … See more To determine the potential of viral RNAs in triggering IL-1β release, different amounts of EV-A71 vRNA were transfected into THP-1 macrophages. The expression … See more birthday gifts for my best girlfriendWebThe effect of the antioxidant compound, N-acetyl-L-cysteine (NAC), on IL-1beta release and regulation of NF-kappaB in a human myelo-monocytic cell line (THP-1) differentiated into … danner duty boots clearance 80 dollarsWebJan 18, 2024 · 不同浓度OMVs作用THP-1细胞时，NLRP3和caspase-1的mRNA表达量较对照组显著升高(P0.05)。作用3 h时，50 μg/mL组NLRP3的mRNA表达量显著高于5 μg/mL … birthday gifts for my boyfriend creative