Toyobo ligation high ver2
WebInstruction manual Ligation high Ver.2 2004 . F1016K . Ligation high Ver.2. LGK-201 750 . μL (100 reactions) Store at -20°C. Contents . Example 3. Example 6. [1] Introduction [2] … WebLigation high Ver.2 is a highly efficient premixed T4 Ligase reagent. Features of Ligation high Ver.2 Highly efficient* Effective ligation of cohesive, blunt and A overhang DNA …
Toyobo ligation high ver2
Did you know?
http://www.bio-toyobo.cn/pdf/KOD-201e.pdf WebNov 8, 2024 · When T cells are resting, they do not make cytokines such as interleukins 2, 4 or 7. Nor do they express large amounts of their receptors. There are no IL-2 receptors. …
http://www.bio-toyobo.cn/product_detail_36.html WebDNA ligation kits, quick DNA Ligation Kit, Version 1 and DNA Ligation Kit, Version 2.1 enable high-efficiency ligation of DNA molecules in vitro. Using an optimized buffer system and T4 DNA Ligase, these DNA ligation kits offer fast ligation in 30 minutes.
WebManufacturer: TOYOBO USA INC LGK201 This product was recently added by customer request, and is available for your convenience. We strive to provide our customers with a … Web(up to 24 kb from human genomic DNA) compared to conventional PCR. -Requires only 30 sec/kb for the PCR extension step. -2-step cycle conditions can be used for amplification using ≥ 20 mer primers (melting temperatures, Tm >63°C). JAPAN TOYOBO CO., LTD. Tel(81)-6-6348-3888 www.toyobo.co.jp/e/bio [email protected]CHINA
Web3 [1] Insert insertion ligation 1. Method Five μl of DNA solution was prepared by adding 1,444 bp fragments (5-125 ng, 5-125 fmol) of pUC18/Taq I to dephosphorated pBluescript II/Ban III (50 ng, 25 fmol). Half (2.5μl) or an equivalent amount (5μl) of "Ligation high" was mixed with DNA solution and this was reacted at 16°C for 30 minutes. The competent cell of E. …
Web製品内容. Ligation high Ver.2 750μL. ※1回の使用量を7.5μLとした場合、100回用として使用できます。. 注意事項. -30℃以下の温度で長期間保存した場合や、冷凍庫内の冷気の … industrial and shippers supply houstonWeb本试剂是以T4 DNA Ligase为主体的高效率单管型连接试剂，广泛应用于各种末端DNA的连接反应。通过对原产品进行改良，可简便地用于各种用途。本试剂是在原产品『Ligation … industrial andon systemWebToyobo ligation high ver 2 Ligation High Ver 2, supplied by Toyobo, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more log cabin urns for human ashesWebToyobo’s hollow-fiber FO membrane used at world’s first*1 osmotic power plant to help bring next-generation renewable energy system into practical use. Oct 18, 2024 Products. … industrial and office relocations seattleWebJan 29, 2024 · The resulting short double strand DNA was subjected to BsaI digestion (New England BioLabs) of pBACi with Ligation high Ver. 2 (Toyobo) and the ligation mixture was subsequently transformed into DH5α. After selection on Luria-Bertani agar supplemented with 10 µg/ml chloramphenicol (Wako Pure Chemical Industries), colony PCR and direct ... log cabin tree skirtWebJan 22, 2015 · Ligation high Ver.2. 750 μl (100 reactions) LGK-201. TArget Clone -Plus- is a high efficient TA cloning kit. The kit can be applied to the TA. cloning of blunt-end PCR products amplified using KOD-Plus- [Code No. KOD-201] or. KOD FX [Code No. KFX-101]. The kit contains pTA2 Vector, 2x Ligation Buffer, T4. DNA Ligase and 10x A-attachment Mix. industrial and product designerWeb2-3. ligation of annealed oligo and gRNA expression plasmid Ligation high (1/2 volume of DNA) 1 ul Bbs ... 1/100 diluted annealed oligo 1 ul / 3 ul e.g. (TOYOBO Ligation high ver.2) 2-4. transformation Add ligated plasmid (~5 ul) to 50 ulof competent cells (e.g. DH5!) Incubate cells on ice, for 30 min Heat at 42˚C, 30 sec Cool down on ice, for ... industrial and systems engineering careers